This graduate course aims to teach the fundamentals of biological mass spectrometry with particular focus on applications to advanced analysis of protein modifications. The course will begin with an introduction to the general aspects of mass spectrometry and proteomic analysis, to give sufficient background knowledge on current advances in topics such as protein identification and quantitative shotgun proteomics. This will then be followed by in depth coverage on the practical aspects of MS analysis of selected protein modifications, each of which will be preceded by an invited lecture on the biological significance and supplemented by paper discussion.
Tentative course content includes :
1) Fundamentals of Biological MS and Proteomics (3 lectures)
2) Phosphorylation (3 lectures)
3) Cysteine modification : Chemical S-Alkylation, Oxidation and S-Nitrosylation (3 lectures)
4) Ubiquitination and Sumoylation Neddylation (2 lectures)
5) Methylation, Acetylation and other Amino-modifications (2 lectures)
6) Glycosylation (3 lectures)